A rapid and efficient method for transcript mapping

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A rapid and efficient method for transcript mapping.

Several methods for transcript mapping using SI nuclease have been described (1, 2). These methods use purified probe and hence involve multistep procedures for probe purification. Here I describe an easy and efficient method for transcript mapping which uses the probe without purification. The method synthesizes the probe by primer-extension and uses this probe directly for RNA:DNA hybridizati...

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a report of rapid and efficient method for mitochondrial dna

based on the similarity of prokaryotic dna to that of mitochondrial dna, a rapid and efficient method for dna purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by marmur (1983) which resulted in a high degree of purity (a260/a280=2). in addition we have applied the same technique for the first time to purify the ...

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Transcript Mapping

The sequence of the 1173 nt 12S kinetoplast ribosomal RHA from TiPJ sfimarpa tarentolae was determined from the maxicircle DNA sequence, and the 5* and 3' ends localized by primer runoff and SI nuclease protection experiments. The gene was shown to be free of introns by SI nuclease analysis. A partial secondary structure model of the 12S RHA molecule is presented which is equivalent in certain ...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1990

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/18.20.6159